10X HDAC Assay Buffer
Quick Overview for 10X HDAC Assay Buffer (ABIN925590)
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Supplier Product No.
- mb-068-0020
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Supplier
- Rockland
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原理
- 10X HDAC Assay Buffer
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纯化方法
- This product was aseptically filtered through a 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.
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过滤
- Sterile filtered
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
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应用备注
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Application Note: This product is a 10X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 10X concentrate with 9 parts distilled-deionized water or equivalent. Diluted (1X) HDAC Assay buffer consists of 25 mM Tris Cl, 137 mM NaCl, 2.7 mM KCl a
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 10 X
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注意事项
- Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature
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储存条件
- -20 °C
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储存方法
- Store HDAC Assay Buffer at -20° C or colder prior to opening. Aliquot and re-freeze for best results. Some salts may precipitate out of solution at lower temperature. Allow buffer to equilibrate to room temperature (18° to 26° C) to restore solubility Expiration date is six (6) months from date of receipt.
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有效期
- 6 months
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背景
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Synonyms: HDAC Activity Assay Kit
Background: Histone deacetylases (HDACs) are typically found in multiprotein complexes and are tightly regulated by subcellular localization, phosphorylation, and likely by other mechanisms. HDAC assays allow the determination of deacetylase activity and provide acc
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